WEIGHT MANAGEMENT PROPERTIES
Weight management is crucial for maintaining overall health and reducing the risk of chronic diseases such as diabetes, heart disease, and hypertension. It also supports better energy levels, mobility, and mental well-being, enhancing overall quality of life.
Functional ingredients such as probiotics, postbiotics, plant extracts and other bioactives are increasingly recognized for their potential to support weight management through diverse biological mechanisms.
They can affect the release of key satiety hormones, influence lipid accumulation and leptin signaling in fat cells, and bind dietary cholesterol and fatty acids in the gut.
Consequently, these products may offer promising strategies for addressing overweight and obesity through natural, targeted approaches.
Weigth Management
REGULATION OF SATIETY HORMONES
-
Functional ingredients such as probiotics, postbiotics, plant extracts and other bioactives are increasingly recognized for their potential to support weight management through influencing the release of key satiety hormones,
STC-1
Differentiation
Incubation with the product
Harvesting Supernatant
Quantification transcription
Quantification hormone secretion
Bioinformatic
& customized visualization
The ASSAY
-
The STC-1 cell line exhibits key characteristics of native intestinal enteroendocrine cells and is a well-established in vitro model for studying the expression and secretion of satiety-related hormones such as GIP, CCK, GLP-1, and PYY.
-
Probiotics, postbiotics, and functional ingredients can modulate the release of these hormones, influencing appetite regulation.
-
In this assay, differentiated STC-1 cells are incubated with the test product(s). To characterize the product’s appetite-modulating properties, hormone secretion is quantified by ELISA and gene expression can be assessed by qRT-PCR.
LIPID ACCUMULATION & LEPTIN RELEASE
-
Adipocytes play a crucial role in weight management and participate in long-term appetite regulation. The lipids that accumulate in adipocytes serve as the body's primary energy storage. However, excessive accumulation can lead to obesity, metabolic dysfunction, and insulin resistance.
-
Adipocytes also produce leptin, a hormone signaling the brain to reduce food intake and increase energy expenditure when fat stores are sufficient.
The ASSAY
-
The 3T3-L1 cell line can be differentiated from preadipocytes into mature adipocytes and is widely used as an in vitro model for adipogenesis.
-
Postbiotics, probiotics, and functional ingredients may influence both lipid accumulation and the secretion of leptin, a key adipokine involved in appetite regulation.
-
In this assay, differentiated 3T3-L1 adipocytes are incubated with the test product(s). Lipid accumulation is assessed by Oil Red O staining. Optionally, the culture supernatant can be collected for leptin quantification.
3T3-L1 cells
Differentiation
(3 weeks)
Incubation with the product
Quantification leptin secretion
Staining with Oil Red O
Harvesting Supernatant
Quantification lipid accumulation
Bioinformatic
& customized visualization
CHOLESTEROL AND FATTY ACID BINDING
-
High level of triglyceride and/or cholesterol are among the most significant risk factors for cardiovascular disease.
-
Orally taken products may decrease the triglyceride and/or cholesterol level via various mechanisms.
Incubation test Product with cholesterol or fatty acid
Quantification cholesterol or fatty acid
Isolation of supernatants
The ASSAY
-
In this assay, the test compound is incubated with cholesterol or fatty acid. After incubation, the remaining unbound cholesterol or fatty acid in the supernatant is quantified to determine the binding properties of the tested product.
MICROBIOME MODULATION
-
Microbiome modulation can support weight management by influencing metabolism, appetite regulation and fat storage through the production of bioactive compounds and interactions with the gut-brain axis. A balanced gut microbiome may enhance nutrient absorption, reduce inflammation and improve insulin sensitivity, contributing a healthier weight control.
-
Modulating the gut microbiome composition presents an exciting strategy for effective weight management.
-
With the HUMIPLATE platform, we offer a comprehensive analysis of your product’s effect on the microbiome. The supernatant of microbiome cultures can be tested for antimicrobial properties, helping identify its potential to modulate microbiome activity and support optimal weight management.
Have a non-binding consultation with one of our experts to find out the best solution for your needs.
