The Multi-Omic and Functional Analysis Platform
ACARYON's integrative multi-omics and functional platform allows to assess any aspect of the microbiome compound relationship.
- We can analyse any microbiome samples:
Human microbiome samples (gut, skin, vagina etc...)
Animal microbiome samples (mice, rat etc..)
Vegetal microbiome samples (plant, soil, water etc...)
- Our platform allows to integrate analysis aspects of
Intestinal and Immuno Profiling
The Multi-Omic & Functional Platform
Intestinal & Immuno
The functional properties of the microbiome are directly related to its taxonomical composition. Based on qPCR and Next Generation Sequencing the composition of the microbiome can be analyzed at any level.
Quantitative PCR (qPCR) is a first level analysis that allows determining changes in whole bacteria as well as in specific groups of bacteria like Bacteroides. Spp etc…
Species specific qPCR is the tool of choice for quick and affordable detection of changes in one or a restricted number of species over time or in response to treatment.
16S RNA Metagenomic
16S rRNA gene sequencing (16S amplicon sequencing) helps evaluating the relative abundance of main bacterial taxa composing the microbiome. This analysis is dedicated to identifying changes in the overall microbial profile over time, or between treatments.
Our shotgun metagenomics services facilitate detailed taxa profiling with high coverage at species level as well as more advance functional profiling of the microbiome without PCR amplification bias.
ITS2 rRNA Sequencing
ITS2 rRNA gene sequencing (ITS2 amplicon sequencing) helps evaluating the relative abundance of main fungal taxa in the microbiome. This analysis is dedicated to identifying changes in the overall microbial profile over time or between treatments.
Functional profiling or functional predictive profiling aims to go one step beyond the composition and to directly identify enzymatic functions present in the microbiome. Recent years have seen advances in linking microbiome sequencing data to metabolome data. These linkages are used to infer molecular compound identities from the genetic information housed within a microbial community.
16S RNA Predictive Functional Profiling (PFP)
This approach combines taxonomic information from 16S rRNA gene data with functional information (e.g., metabolic pathways) derived from closely related taxa with sequenced genomes.
PFP is a powerful and cost-efficient alternative to shotgun metagenomics for the human gut microbiome
Shotgun metatranscriptomics allows determining microbial activity, differential expression of genes across species, and active metabolic pathways.
Liquid chromatography–mass spectrometry (LC–MS) allows the analysis of specific protein content.
The microbiome generates a variety of metabolites that influence the composition of the microbiome as well as the host’s health and pathophysiological functions.
As the vast majority of microbial taxa belonging to the human gut microbiome remain unknown, metabolite analysis allows to bypass the unknown between tax composition and metabolite production.
Based on LC-MS, we provide a wide range of metabolic analysis including content of short chain fatty acids, vitamins, bile salt metabolites, neurotransmitters etc..
Short chain fatty acids
SCFAs like propionate, butyrate and acetate are mainly produced by the microbiome and are involved in regulating the composition of the microbiome as wells as the host‘s immunity and metabolisms.
Bile salt metabolites
Primary bile acids are further metabolized by the gut microbiota to secondary bile acids. These metabolites strongly impact the gut barrier function and the immune system.
The microbiome can produce several neurotransmitters (e.g., acetylcholine, GABA, dopamine, and serotonin) and modulate the metabolism of others.
The microbiome is directly involved in the production of vitamines like vitamine B12 and vitamine .
Intestinal & Immuno Profiling
The microbiome and substances that it is producing (like polysaccharides, metabolites etc.) directly influence the functions of the immune system.
The Microbiome impacts the immune system through several complementary mechanisms. Thus, the microbiome affects the integrity of the intestinal barrier, modulates the local and systemic immune responses and impacts the colonization of pathogens.
These complex mechanisms may include:
- induction and/or suppression of cytokines, chemokines
- induction of and antimicrobial peptides
- recruitment or activation of cell populations in the gut mucosa
- stimulation of mucosal antibody production
- enhancement of epithelial repair and barrier functions etc.
Based on different cellular intestinal models (human intestinal cell lines Caco2, HT29 or HT29-MTX) we analyze the effect of your products on the microbiome’s cellular and immunomodulatory properties.
The microbiome directly supports the immune system through avoiding growth and colonization of pathogen microorganisms. Two of the main mechanisms are:
- the production of antimicrobial substances
- limiting the binding of pathogens to intestinal cells.
Gut Barrier Integrity
The microbiome is strongly involved in regulating the gut barrier integrity. Thus, the microbiome impacts:
- the mucus production
- The permeability of the gut wall
- the proliferation/differentiation of intestinal cells.
The microbiome is in continuous cross-talk with the immune system. This complex network of innate and adaptive components has to be kept in balance to ensure defence against harmful external agents and to avoid aberrant immune responses that may lead to a variety of diseases.
Permeability of the Gut